产品货号：
GS2094
中文名称：
BstSNI内切酶
英文名称：
BstSNI
产品规格：
200U
发货周期：
1～3天
产品价格：
询价
储存条件：冷冻（-20℃）
使用说明
High enzyme concentration results in star activity
技术规格
相关搜索：BstSNI内切酶，BstSNI限制性内切酶
使用说明
High enzyme concentration results in star activity
技术规格
| Unit definition | One unit of the enzyme is the amount required to hydrolyze 1μgof T7 DNA in 1hourat 37℃ in a total reaction volume of 50μl. |
| Recognition sites | TAC↑GTA |
| 来源 | Bacillus stearothermophilus SN |
| Assayed on | T7 DNA |
| Optimal buffer | B?(10mMTris-HCl(pH 7.6 at 25℃);10mMMgCl2;1 mM DTT.) |
| Optimal temperature | 37℃ |
| Storage conditions | 10mMTris-HCl(pH 7.5);250 mM NaCl;0.1 mM EDTA;100 μg/ml BSA;7 mM 2-mercaptoethanol;50%glycerol.Store at 20℃. |
| Ligations | After 5-fold overdigestion with enzyme about 70%of the DNA fragments can be ligated and recut. |
| Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1μgof T7 DNA with 5 u.a.of enzyme for 16hoursat 37℃. |
| Methlation effect | Methylation sensitivity not tested |
| Thermal inactivation | enzyme is inactivated by incubation at 80℃ for 20 mins. |
| Campatible Ends | Eco105I,SnaBI |
相关搜索：BstSNI内切酶，BstSNI限制性内切酶